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magna rip rna binding protein immunoprecipitation kit  (Merck & Co)

 
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    Structured Review

    Merck & Co magna rip rna binding protein immunoprecipitation kit
    MALAT1 binds to HADHB (A) Schematic representation of the RNA <t>immunoprecipitation</t> <t>(RIP)</t> of LPS-stimulated THP-1-derived macrophages differentiated with PMA for 24 h. (B) Western blot of RIP samples using an anti -HADHB antibody, with IgG as a negative control. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (C) Top ten candidate non-coding RNAs binding to HADHB, ranked by peak score from RIP-seq analysis. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (D) Fold enrichment of MALAT1 in RIP samples. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days); data are presented as mean ± SD and analyzed with the two-tailed unpaired Student’s t test.
    Magna Rip Rna Binding Protein Immunoprecipitation Kit, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/magna+rip+kit/assay+bca+kit+protein/pmc12972699-54-0-7
    Average 86 stars, based on 1 article reviews
    magna rip rna binding protein immunoprecipitation kit - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "MALAT1 regulates human macrophage metabolism by interacting with HADHB"

    Article Title: MALAT1 regulates human macrophage metabolism by interacting with HADHB

    Journal: iScience

    doi: 10.1016/j.isci.2026.115107

    MALAT1 binds to HADHB (A) Schematic representation of the RNA immunoprecipitation (RIP) of LPS-stimulated THP-1-derived macrophages differentiated with PMA for 24 h. (B) Western blot of RIP samples using an anti -HADHB antibody, with IgG as a negative control. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (C) Top ten candidate non-coding RNAs binding to HADHB, ranked by peak score from RIP-seq analysis. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (D) Fold enrichment of MALAT1 in RIP samples. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days); data are presented as mean ± SD and analyzed with the two-tailed unpaired Student’s t test.
    Figure Legend Snippet: MALAT1 binds to HADHB (A) Schematic representation of the RNA immunoprecipitation (RIP) of LPS-stimulated THP-1-derived macrophages differentiated with PMA for 24 h. (B) Western blot of RIP samples using an anti -HADHB antibody, with IgG as a negative control. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (C) Top ten candidate non-coding RNAs binding to HADHB, ranked by peak score from RIP-seq analysis. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (D) Fold enrichment of MALAT1 in RIP samples. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days); data are presented as mean ± SD and analyzed with the two-tailed unpaired Student’s t test.

    Techniques Used: RNA Immunoprecipitation, Derivative Assay, Western Blot, Negative Control, Binding Assay, Two Tailed Test

    Related Articles

    Lysis:

    Article Title: YTHDF1 Regulates RELA m6A Modification and Activates the NF ‐Kappa B Signaling Pathway to Promote the Mechanism of Gastric Cancer
    Article Snippet: RIP assays were conducted using a Magna RIP RNA‐Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer's instructions. .. A total of 1 × 10 7 HGC‐27 cells were harvested and lysed in RIP lysis buffer containing protease inhibitor cocktail and RNA inhibitor; the supernatants were collected after centrifugation for 10 min at 13000 rpm, and then incubated with Dynabeads protein G conjugated with anti‐IgG or anti‐AGO2 using a Magna RIP kit (Merck, USA). ..

    Article Title: YTHDF1 Regulates RELA m6A Modification and Activates the NF-Kappa B Signaling Pathway to Promote the Mechanism of Gastric Cancer.
    Article Snippet: RIP assays were conducted using a Magna RIP RNA- Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer's instructions. .. A total of 1 × 107 HGC- 27 cells were harvested and lysed in RIP lysis buffer containing protease inhibitor cocktail and RNA inhibitor; the supernatants were collected after centrifugation for 10 min at 13000 rpm, and then incubated with Dynabeads protein G conjugated with anti- IgG or anti- AGO2 using a Magna RIP kit (Merck, USA). ..

    Article Title: circHERC1—A telomerase activator
    Article Snippet: RIP assays ( ) were conducted using a Magna RIP kit (Millipore, USA) according to the manufacturer’s instructions. .. A total of 1 × 10 7 HeLa or 293FT cells were harvested and lysed in RIP lysis buffer and then incubated with Dynabeads protein G conjugated with anti–immunoglobulin G (IgG), anti–c-Fos, and anti–RNA polymerase II using a Magna RIP kit (Merck, USA). ..

    Protease Inhibitor:

    Article Title: YTHDF1 Regulates RELA m6A Modification and Activates the NF ‐Kappa B Signaling Pathway to Promote the Mechanism of Gastric Cancer
    Article Snippet: RIP assays were conducted using a Magna RIP RNA‐Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer's instructions. .. A total of 1 × 10 7 HGC‐27 cells were harvested and lysed in RIP lysis buffer containing protease inhibitor cocktail and RNA inhibitor; the supernatants were collected after centrifugation for 10 min at 13000 rpm, and then incubated with Dynabeads protein G conjugated with anti‐IgG or anti‐AGO2 using a Magna RIP kit (Merck, USA). ..

    Article Title: YTHDF1 Regulates RELA m6A Modification and Activates the NF-Kappa B Signaling Pathway to Promote the Mechanism of Gastric Cancer.
    Article Snippet: RIP assays were conducted using a Magna RIP RNA- Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer's instructions. .. A total of 1 × 107 HGC- 27 cells were harvested and lysed in RIP lysis buffer containing protease inhibitor cocktail and RNA inhibitor; the supernatants were collected after centrifugation for 10 min at 13000 rpm, and then incubated with Dynabeads protein G conjugated with anti- IgG or anti- AGO2 using a Magna RIP kit (Merck, USA). ..

    Centrifugation:

    Article Title: YTHDF1 Regulates RELA m6A Modification and Activates the NF ‐Kappa B Signaling Pathway to Promote the Mechanism of Gastric Cancer
    Article Snippet: RIP assays were conducted using a Magna RIP RNA‐Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer's instructions. .. A total of 1 × 10 7 HGC‐27 cells were harvested and lysed in RIP lysis buffer containing protease inhibitor cocktail and RNA inhibitor; the supernatants were collected after centrifugation for 10 min at 13000 rpm, and then incubated with Dynabeads protein G conjugated with anti‐IgG or anti‐AGO2 using a Magna RIP kit (Merck, USA). ..

    Article Title: YTHDF1 Regulates RELA m6A Modification and Activates the NF-Kappa B Signaling Pathway to Promote the Mechanism of Gastric Cancer.
    Article Snippet: RIP assays were conducted using a Magna RIP RNA- Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer's instructions. .. A total of 1 × 107 HGC- 27 cells were harvested and lysed in RIP lysis buffer containing protease inhibitor cocktail and RNA inhibitor; the supernatants were collected after centrifugation for 10 min at 13000 rpm, and then incubated with Dynabeads protein G conjugated with anti- IgG or anti- AGO2 using a Magna RIP kit (Merck, USA). ..

    Incubation:

    Article Title: YTHDF1 Regulates RELA m6A Modification and Activates the NF ‐Kappa B Signaling Pathway to Promote the Mechanism of Gastric Cancer
    Article Snippet: RIP assays were conducted using a Magna RIP RNA‐Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer's instructions. .. A total of 1 × 10 7 HGC‐27 cells were harvested and lysed in RIP lysis buffer containing protease inhibitor cocktail and RNA inhibitor; the supernatants were collected after centrifugation for 10 min at 13000 rpm, and then incubated with Dynabeads protein G conjugated with anti‐IgG or anti‐AGO2 using a Magna RIP kit (Merck, USA). ..

    Article Title: YTHDF1 Regulates RELA m6A Modification and Activates the NF-Kappa B Signaling Pathway to Promote the Mechanism of Gastric Cancer.
    Article Snippet: RIP assays were conducted using a Magna RIP RNA- Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer's instructions. .. A total of 1 × 107 HGC- 27 cells were harvested and lysed in RIP lysis buffer containing protease inhibitor cocktail and RNA inhibitor; the supernatants were collected after centrifugation for 10 min at 13000 rpm, and then incubated with Dynabeads protein G conjugated with anti- IgG or anti- AGO2 using a Magna RIP kit (Merck, USA). ..

    Article Title: circHERC1—A telomerase activator
    Article Snippet: RIP assays ( ) were conducted using a Magna RIP kit (Millipore, USA) according to the manufacturer’s instructions. .. A total of 1 × 10 7 HeLa or 293FT cells were harvested and lysed in RIP lysis buffer and then incubated with Dynabeads protein G conjugated with anti–immunoglobulin G (IgG), anti–c-Fos, and anti–RNA polymerase II using a Magna RIP kit (Merck, USA). ..

    Reporter Assay:

    Article Title: m 6 A-driven SF3B1 translation control steers splicing to direct genome integrity and leukemogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Dual-Luciferase Reporter Assay System Promega CAT# E1970 mMESSAGE mMACHINE T7 Transcription Kit Ambion CAT# AM1344 Tobacco Acid Phosphatase Thermo Fisher Scientific CAT# AM1700M Magna RIP kit MERCK CAT# 17-700 Transcription Factor Buffer Set BD Biosciences CAT#562574 Alt-R S. p. CRISPR-Cas9 guide RNA kit Integrated DNA Technologies CAT#1081060 CometSlide R&D Systems CAT#4250-200-03 b-mercaptoethanol Gibco CAT#31350010 SE Cell Line 4D-NucleofectorTM X Kit L Lonza CAT#V4XC-1012 FirstChoice RLM-RACE Kit Invitrogen CAT#AM1700 MethoCult H4434 Classic Stemcell Technologies CAT#04434 MethoCult GF M3434 Stemcell Technologies CAT#03434 Deposited data Raw and analyzed data: ASE-seq human fibroblasts This paper GEO: GSE189585 Raw and analyzed data: DMS probing This paper GEO: GSE147504 Raw and analyzed data: NHD13 cKIT+ RNA-seq This paper GEO: GSE198464 Raw and analyzed data: MOLM13 RNA-seq This paper GEO: GSE189584 Raw data Mendeley Dataset This paper https://data.mendeley.com/datasets/ftfvj7ct7f Experimental models: Cell lines WI-38 hT TRE-MYC This paper N/A WI-38 hT TRE-MYC This paper N/A WI-38 hT TRE-MYC SF3B1 5’UTR WT MS2 This paper N/A WI-38 hT TRE-MYC SF3B1 5’UTR A88G MS2 This paper N/A ..

    CRISPR:

    Article Title: m 6 A-driven SF3B1 translation control steers splicing to direct genome integrity and leukemogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Dual-Luciferase Reporter Assay System Promega CAT# E1970 mMESSAGE mMACHINE T7 Transcription Kit Ambion CAT# AM1344 Tobacco Acid Phosphatase Thermo Fisher Scientific CAT# AM1700M Magna RIP kit MERCK CAT# 17-700 Transcription Factor Buffer Set BD Biosciences CAT#562574 Alt-R S. p. CRISPR-Cas9 guide RNA kit Integrated DNA Technologies CAT#1081060 CometSlide R&D Systems CAT#4250-200-03 b-mercaptoethanol Gibco CAT#31350010 SE Cell Line 4D-NucleofectorTM X Kit L Lonza CAT#V4XC-1012 FirstChoice RLM-RACE Kit Invitrogen CAT#AM1700 MethoCult H4434 Classic Stemcell Technologies CAT#04434 MethoCult GF M3434 Stemcell Technologies CAT#03434 Deposited data Raw and analyzed data: ASE-seq human fibroblasts This paper GEO: GSE189585 Raw and analyzed data: DMS probing This paper GEO: GSE147504 Raw and analyzed data: NHD13 cKIT+ RNA-seq This paper GEO: GSE198464 Raw and analyzed data: MOLM13 RNA-seq This paper GEO: GSE189584 Raw data Mendeley Dataset This paper https://data.mendeley.com/datasets/ftfvj7ct7f Experimental models: Cell lines WI-38 hT TRE-MYC This paper N/A WI-38 hT TRE-MYC This paper N/A WI-38 hT TRE-MYC SF3B1 5’UTR WT MS2 This paper N/A WI-38 hT TRE-MYC SF3B1 5’UTR A88G MS2 This paper N/A ..

    RNA Sequencing:

    Article Title: m 6 A-driven SF3B1 translation control steers splicing to direct genome integrity and leukemogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Dual-Luciferase Reporter Assay System Promega CAT# E1970 mMESSAGE mMACHINE T7 Transcription Kit Ambion CAT# AM1344 Tobacco Acid Phosphatase Thermo Fisher Scientific CAT# AM1700M Magna RIP kit MERCK CAT# 17-700 Transcription Factor Buffer Set BD Biosciences CAT#562574 Alt-R S. p. CRISPR-Cas9 guide RNA kit Integrated DNA Technologies CAT#1081060 CometSlide R&D Systems CAT#4250-200-03 b-mercaptoethanol Gibco CAT#31350010 SE Cell Line 4D-NucleofectorTM X Kit L Lonza CAT#V4XC-1012 FirstChoice RLM-RACE Kit Invitrogen CAT#AM1700 MethoCult H4434 Classic Stemcell Technologies CAT#04434 MethoCult GF M3434 Stemcell Technologies CAT#03434 Deposited data Raw and analyzed data: ASE-seq human fibroblasts This paper GEO: GSE189585 Raw and analyzed data: DMS probing This paper GEO: GSE147504 Raw and analyzed data: NHD13 cKIT+ RNA-seq This paper GEO: GSE198464 Raw and analyzed data: MOLM13 RNA-seq This paper GEO: GSE189584 Raw data Mendeley Dataset This paper https://data.mendeley.com/datasets/ftfvj7ct7f Experimental models: Cell lines WI-38 hT TRE-MYC This paper N/A WI-38 hT TRE-MYC This paper N/A WI-38 hT TRE-MYC SF3B1 5’UTR WT MS2 This paper N/A WI-38 hT TRE-MYC SF3B1 5’UTR A88G MS2 This paper N/A ..

    RNA Immunoprecipitation:

    Article Title: Methyltransferase-like 14 mediated FOXP1 m6A modification alleviates osteoporosis by regulating the Wnt/β-catenin pathway.
    Article Snippet: are a specific population of stem cells that are essential for skeletal development and bone regeneration (Lin et al. 2019).. Impaired osteogenic differentiation of BMSCs is closely linked to the progression of OP (Chen et al. 2019; Ning et al. 2022).. This decline in osteogenic potential can exacerbate local inflammation, perturb bone remodeling, and disrupt bone-fat homeostasis, all of which contribute to the development of OP (Cheng et al. 2023).



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    Merck & Co magna rip rna binding protein immunoprecipitation kit
    MALAT1 binds to HADHB (A) Schematic representation of the RNA <t>immunoprecipitation</t> <t>(RIP)</t> of LPS-stimulated THP-1-derived macrophages differentiated with PMA for 24 h. (B) Western blot of RIP samples using an anti -HADHB antibody, with IgG as a negative control. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (C) Top ten candidate non-coding RNAs binding to HADHB, ranked by peak score from RIP-seq analysis. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (D) Fold enrichment of MALAT1 in RIP samples. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days); data are presented as mean ± SD and analyzed with the two-tailed unpaired Student’s t test.
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    MALAT1 binds to HADHB (A) Schematic representation of the RNA <t>immunoprecipitation</t> <t>(RIP)</t> of LPS-stimulated THP-1-derived macrophages differentiated with PMA for 24 h. (B) Western blot of RIP samples using an anti -HADHB antibody, with IgG as a negative control. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (C) Top ten candidate non-coding RNAs binding to HADHB, ranked by peak score from RIP-seq analysis. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (D) Fold enrichment of MALAT1 in RIP samples. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days); data are presented as mean ± SD and analyzed with the two-tailed unpaired Student’s t test.
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    MALAT1 binds to HADHB (A) Schematic representation of the RNA <t>immunoprecipitation</t> <t>(RIP)</t> of LPS-stimulated THP-1-derived macrophages differentiated with PMA for 24 h. (B) Western blot of RIP samples using an anti -HADHB antibody, with IgG as a negative control. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (C) Top ten candidate non-coding RNAs binding to HADHB, ranked by peak score from RIP-seq analysis. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (D) Fold enrichment of MALAT1 in RIP samples. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days); data are presented as mean ± SD and analyzed with the two-tailed unpaired Student’s t test.
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    Image Search Results


    MALAT1 binds to HADHB (A) Schematic representation of the RNA immunoprecipitation (RIP) of LPS-stimulated THP-1-derived macrophages differentiated with PMA for 24 h. (B) Western blot of RIP samples using an anti -HADHB antibody, with IgG as a negative control. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (C) Top ten candidate non-coding RNAs binding to HADHB, ranked by peak score from RIP-seq analysis. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (D) Fold enrichment of MALAT1 in RIP samples. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days); data are presented as mean ± SD and analyzed with the two-tailed unpaired Student’s t test.

    Journal: iScience

    Article Title: MALAT1 regulates human macrophage metabolism by interacting with HADHB

    doi: 10.1016/j.isci.2026.115107

    Figure Lengend Snippet: MALAT1 binds to HADHB (A) Schematic representation of the RNA immunoprecipitation (RIP) of LPS-stimulated THP-1-derived macrophages differentiated with PMA for 24 h. (B) Western blot of RIP samples using an anti -HADHB antibody, with IgG as a negative control. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (C) Top ten candidate non-coding RNAs binding to HADHB, ranked by peak score from RIP-seq analysis. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (D) Fold enrichment of MALAT1 in RIP samples. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days); data are presented as mean ± SD and analyzed with the two-tailed unpaired Student’s t test.

    Article Snippet: Magna RIP RNA-Binding Protein Immunoprecipitation Kit , Merck , Cat# 17-700.

    Techniques: RNA Immunoprecipitation, Derivative Assay, Western Blot, Negative Control, Binding Assay, Two Tailed Test